There are two references to Spider-Man co-creator Steve Ditko. The first is that Peter Parker's landlord is called Mr DITKOvich. The second is that while Hoffman and Jameson are thinking up titles for the new villain, Hoffman suggests "Dr Strange", a superhero who was also co-created by Steve Ditko.

In the scene where Spider-Man and Doc Ock are fighting on the clock tower, Doc Ock throws a clock hand at Spider-Man and it falls to the ground. When it hits the ground, we see two people moving out of the way to avoid being hit. One of the them happens to be the creator of Spider-Man, Stan Lee.